不是说没证据吗? 这是实锤
https://www.nature.com/articles/nm.3985 6park.comhttps://www.nature.com/articles/nm.3985.pdf 6park.com2015年发表的文章,还怎么抵赖呢? 6park.com五毛看不懂英文的,去问问你们主子的主子的主子,看是不是有这个事情 6park.comA SARS-like cluster of circulating bat coronaviruses shows potential for human emergence
Vineet D Menachery, Boyd L Yount Jr, Kari Debbink, Sudhakar Agnihothram, Lisa E Gralinski, Jessica A Plante, Rachel L Graham, Trevor Scobey, Xing-Yi Ge, Eric F Donaldson, Scott H Randell, Antonio Lanzavecchia, Wayne A Marasco, Zhengli-Li Shi & Ralph S Baric
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Nature Medicine 6park.com volume 21, pages1508–1513(2015)Cite this article
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A Corrigendum to this article was published on 06 April 2016
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Abstract
The
emergence of severe acute respiratory syndrome coronavirus (SARS-CoV)
and Middle East respiratory syndrome (MERS)-CoV underscores the threat
of cross-species transmission events leading to outbreaks in humans.
Here we examine the disease potential of a SARS-like virus, SHC014-CoV,
which is currently circulating in Chinese horseshoe bat populations1. Using the SARS-CoV reverse genetics system2,
we generated and characterized a chimeric virus expressing the spike of
bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone. The
results indicate that group 2b viruses encoding the SHC014 spike in a
wild-type backbone can efficiently use multiple orthologs of the SARS
receptor human angiotensin converting enzyme II (ACE2), replicate
efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo
experiments demonstrate replication of the chimeric virus in mouse lung
with notable pathogenesis. Evaluation of available SARS-based
immune-therapeutic and prophylactic modalities revealed poor efficacy;
both monoclonal antibody and vaccine approaches failed to neutralize and
protect from infection with CoVs using the novel spike protein. On the
basis of these findings, we synthetically re-derived an infectious
full-length SHC014 recombinant virus and demonstrate robust viral
replication both in vitro and in vivo. Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.
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